ETTO's medicinal grade Tea Tree oil has a super high percentage of the component terpinen-4-ol which is a potent naturally occurring bioactive that has been shown to effectively reduce the hatching of lice eggs and can kill lice and nits. We recommend spraying school hats, brushes and combs, and the ends of hair with The Pure Oil Spray to prevent an outbreak as the smell of eucalyptus and tea tree alone deters insects. As a natural solution, use ETTO's pure Tea Tree oil with a carrier oil to use as a scalp treatment and comb through with a nit comb. Repeat as needed.